KMID : 1007520220310101277
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Food Science and Biotechnology 2022 Volume.31 No. 10 p.1277 ~ p.1287
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Impact of different extraction conditions on yield, physicochemical and functional characteristics of gelatin from Labeo rohita swim bladder
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Kanwate Balaji Wamanrao
Kudre Tanaji G.
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Abstract
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Influences of different extraction temperatures (50, 60, and 70 ¡ÆC) and time (3, 6, and 9 h) on yield, physicochemical, and functional properties of gelatin from swim bladder (SBG) of rohu (Labeo rohita) were investigated. Gelatin extracted at 60 ¡ÆC (G60-9 h), and 70 ¡ÆC (G70-9 h) temperature for 9 h showed highest gelatin yield 54.91 g/100 g, protein (92.35 g/100 g), and hydroxyproline (77.50 mg/g) content (P?0.05) compared to other counterparts. All extracted gelatin showed major protein components as ¥á-, ¥â-, and ¥ã-chain with slight changes in band intensity. FTIR spectra showed significant loss of molecular order of triple-helix as temperature and time increased. Furthermore, G60-9 h exhibited higher emulsion, foaming, and gel strength than G70-9 h, and G50-9 h, respectively (P?0.05). Therefore, G60-9 h could be appropriate conditions for the extraction of SBG with better yield, physicochemical, and functional properties.
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KEYWORD
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Labeo rohita swim bladder gelatin, Extraction temperature, Extraction time, Physicochemical characteristics, Functional properties
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